This tutorial explores how a darkfield microscopy specimen scatters light into the objective when illuminated with a hollow cone of oblique illumination from a Cardioid darkfield condenser.
To operate the tutorial, move the slider back and forth and watch the sample move into place in front of the objective. As the sample encounters light from the Cardioid condenser, it refracts, diffracts, and scatters the light into the objective. The physical concepts behind this tutorial do not rely specifically on the Cardioid-type condenser, and the same principles apply to all darkfield condensers.
Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.
Robert T. Sutter, John C. Long, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.
BACK TO DARKFIELD MICROSCOPY
Questions or comments? Send us an email.
© 1998-2015 by
Michael W. Davidson and The Florida State University.
All Rights Reserved. No images, graphics, scripts, or applets may be reproduced or used in any manner without permission from the copyright holders. Use of this website means you agree to all of the Legal Terms and Conditions set forth by the owners.
Last modification: Thursday, Feb 25, 2016 at 04:59 PM
Access Count Since July 30, 1998: 49649
For more information on microscope manufacturers,
use the buttons below to navigate to their websites: