Jablonski Diagram
Laser
400
500
600
700
800
900
1000
1097nm
Higher energy and vibration states
Lowest singlet excited state
Excitation599 nm
Emission674 nm
Ground state
Single Photon Excitation
Delay: 10-20 sec
4
3
2
1
0
2
1
0
IncidentPhoton
Excitation10-20 s
Relaxation10-20 s
Fluorescence
10-20 s
Excitation Delay
Start