Birefringent Regions in Cholesteric Mesophases

Birefringent Regions in Cholesteric Mesophases

Birefringent regions of indeterminate structure occur in the cholesteric mesophase of liquid crystalline DNA samples exposed to high magnetic fields. The photomicrograph presented above illustrates a region having strong birefringence and exhibiting a wide spectrum of higher order colors, which may be due to thickness variations. The magnification is approximately 75x, and the image was recorded on Fujichrome 64T transparency film using a Nikon Optiphot-Pol microscope with crossed polarized illumination. Exposures were recorded about 3 f-steps under the recommended value given by an in-camera photomultiplier and were push-processed approximately 1.5 f-steps in the first E-6 developer.

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