Spherulitic Domains in Cholesteric DNA Mesophases

Spherulitic Domains in Cholesteric DNA Mesophases

Spherulitic cholesteric domains appear in this image as birefringent spheres with blue and yellow interference colors generated by a full-wave retardation plate. The DNA concentration for this specimen was approximately 200 milligrams per millimeter, but this changes as the solvent evaporates in controlled drying experiments. The magnification is approximately 350x. Originally recorded on Fujichrome 64T transparency film using a Nikon Optiphot-Pol microscope with crossed polarized illumination, the image above was digitized using a Nikon CoolScan transparency film scanner. Exposures were recorded about 2.5 f-steps under the recommended value given by an in-camera photomultiplier and were push-processed approximately 1.5 f-steps in the first E-6 developer.

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